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Addgene

pNHA7
(Plasmid #223649)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 223649 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pQE80L
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BioID_G118R
  • Alt name
    birA
  • Species
    Synthetic
  • Mutation
    G118R
  • Promoter LacI
  • Tag / Fusion Protein
    • 6His (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNHA7 was a gift from François-Xavier Campbell-Valois (Addgene plasmid # 223649 ; http://n2t.net/addgene:223649 ; RRID:Addgene_223649)
  • For your References section:

    The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC in Shigella flexneri. Haidar-Ahmad N, Tomaro K, Lavallée-Adam M, Campbell-Valois F. 0.. mSphere 0:e00553-24 10.1128/msphere.00553-24