pNHA7
(Plasmid
#223649)
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PurposebirA protein purification
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepQE80L
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBioID_G118R
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Alt namebirA
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SpeciesSynthetic
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MutationG118R
- Promoter LacI
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Tag
/ Fusion Protein
- 6His (N terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer TGC TTT GTG AGC GGA TAA C (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNHA7 was a gift from François-Xavier Campbell-Valois (Addgene plasmid # 223649 ; http://n2t.net/addgene:223649 ; RRID:Addgene_223649) -
For your References section:
The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC in Shigella flexneri. Haidar-Ahmad N, Tomaro K, Lavallée-Adam M, Campbell-Valois F. 0.. mSphere 0:e00553-24 10.1128/msphere.00553-24