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pCI-MOR222-1
(Plasmid #22363)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4006
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MOR222-1
  • Alt name
    Mus musculus olfactory receptor 312
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    927
  • GenBank ID
    NM_146537
  • Entrez Gene
    Olfr312 (a.k.a. MGC129174, MGC129181, MOR222-4P, GA_x5J8B7W3UM0-1747565-1747342)
  • Tag / Fusion Protein
    • Rho (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This clone was originally identified as Olfr311, but upon resequencing, it is actually Olfr312.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-MOR222-1 was a gift from Hiroaki Matsunami (Addgene plasmid # 22363 ; http://n2t.net/addgene:22363 ; RRID:Addgene_22363)

  • For your References section:

    Odor coding by a Mammalian receptor repertoire. Saito H, Chi Q, Zhuang H, Matsunami H, Mainland JD. Sci Signal. 2009 . 2(60):ra9. 10.1126/scisignal.2000016 PubMed 19261596
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