pMSCV_FLT3
(Plasmid
#223566)
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PurposeThe plasmid is expressed FLT3 in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCV
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLT3 wild type
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Alt nameFLT3
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SpeciesH. sapiens (human)
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Insert Size (bp)2982
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Entrez GeneFLT3 (a.k.a. CD135, FLK-2, FLK2, STK1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pLXSN5'
- 3′ sequencing primer MSCV_C_rev2 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAfter purchasing Addgene plasmid #74498 (deposited by Lars Rönnstrand), proceeded with mutation and cloning.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV_FLT3 was a gift from Hyun Woo Park (Addgene plasmid # 223566 ; http://n2t.net/addgene:223566 ; RRID:Addgene_223566) -
For your References section:
Targeting FLT3-TAZ signaling to suppress drug resistance in blast phase chronic myeloid leukemia. Shin JE, Kim SH, Kong M, Kim HR, Yoon S, Kee KM, Kim JA, Kim DH, Park SY, Park JH, Kim H, No KT, Lee HW, Gee HY, Hong S, Guan KL, Roe JS, Lee H, Kim DW, Park HW. Mol Cancer. 2023 Nov 6;22(1):177. doi: 10.1186/s12943-023-01837-4. 10.1186/s12943-023-01837-4 PubMed 37932786