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Addgene

pmCherry-Synaptojanin-1-145
(Plasmid #223563)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223563 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmCherry-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SYNJ1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SYNJ1 (a.k.a. DEE53, EIEE53, INPP5G, PARK20)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • mCherry (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-Synaptojanin-1-145 was a gift from Pietro De Camilli (Addgene plasmid # 223563 ; http://n2t.net/addgene:223563 ; RRID:Addgene_223563)
  • For your References section:

    Parkinsonism Sac domain mutation in Synaptojanin-1 affects ciliary properties in iPSC-derived dopaminergic neurons. Mohd Rafiq N, Fujise K, Rosenfeld MS, Xu P, De Camilli P. Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2318943121. doi: 10.1073/pnas.2318943121. Epub 2024 Apr 18. 10.1073/pnas.2318943121 PubMed 38635628