pmCherry-Synaptojanin-1-145
(Plasmid
#223563)
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PurposeExpresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223563 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYNJ1
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SpeciesH. sapiens (human)
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Entrez GeneSYNJ1 (a.k.a. DEE53, EIEE53, INPP5G, PARK20)
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Tags
/ Fusion Proteins
- His (N terminal on insert)
- mCherry (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCherry-Synaptojanin-1-145 was a gift from Pietro De Camilli (Addgene plasmid # 223563 ; http://n2t.net/addgene:223563 ; RRID:Addgene_223563) -
For your References section:
Parkinsonism Sac domain mutation in Synaptojanin-1 affects ciliary properties in iPSC-derived dopaminergic neurons. Mohd Rafiq N, Fujise K, Rosenfeld MS, Xu P, De Camilli P. Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2318943121. doi: 10.1073/pnas.2318943121. Epub 2024 Apr 18. 10.1073/pnas.2318943121 PubMed 38635628