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Addgene

pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A
(Plasmid #223560)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223560 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-IRES-Hygro
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc
  • Alt name
    Gαi bONE-GO W139A
  • Species
    Synthetic
  • Insert Size (bp)
    3130
  • Mutation
    W139A mutation in GINIP sequence
  • Promoter CMV

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A was a gift from Mikel Garcia-Marcos (Addgene plasmid # 223560 ; http://n2t.net/addgene:223560 ; RRID:Addgene_223560)

  • For your References section:

    A sensitive biosensor of endogenous Galpha(i) activity enables the accurate characterization of endogenous GPCR agonist responses. Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M. Sci Signal. 2025 Mar 25;18(879):eadp6457. doi: 10.1126/scisignal.adp6457. Epub 2025 Mar 25. 10.1126/scisignal.adp6457 PubMed 40132053
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