YFP-CAAX
(Plasmid
#223558)
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PurposePlasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepVenus-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYFP(Venus) - KRas4b
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Alt nameVenus-kras
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SpeciesSynthetic
Resource Information
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A portion of this plasmid was derived from a plasmid made byReceived from Nevin Lambert, Augusta University, Augusta, GA.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YFP-CAAX was a gift from Mikel Garcia-Marcos (Addgene plasmid # 223558 ; http://n2t.net/addgene:223558 ; RRID:Addgene_223558) -
For your References section:
A sensitive biosensor of endogenous Galpha(i) activity enables the accurate characterization of endogenous GPCR agonist responses. Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M. Sci Signal. 2025 Mar 25;18(879):eadp6457. doi: 10.1126/scisignal.adp6457. Epub 2025 Mar 25. 10.1126/scisignal.adp6457 PubMed 40132053