p3xFLAG-CMV-14-GINIP-Nluc W139A
(Plasmid
#223557)
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PurposePlasmid expressing GINIP-Nluc W139A in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223557 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3xFLAG-CMV-14
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman GINIP W139A - linker(GGGS) - Nluc
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Alt nameGINIP-Nluc W139A
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SpeciesSynthetic
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Insert Size (bp)1806
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MutationW139A mutation in GINIP sequence
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3xFLAG-CMV-14-GINIP-Nluc W139A was a gift from Mikel Garcia-Marcos (Addgene plasmid # 223557 ; http://n2t.net/addgene:223557 ; RRID:Addgene_223557) -
For your References section:
A sensitive biosensor of endogenous Galpha(i) activity enables the accurate characterization of endogenous GPCR agonist responses. Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M. Sci Signal. 2025 Mar 25;18(879):eadp6457. doi: 10.1126/scisignal.adp6457. Epub 2025 Mar 25. 10.1126/scisignal.adp6457 PubMed 40132053