GvpA-CaM
(Plasmid #223555)

• Purpose: Full length gas vesicle coat protein A derived from H. salinarum (GvpA) fused to calmodulin.
• Depositing Lab: Jean Gariepy
• Publication: Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6):1864-1873. doi: 10.1021/acsptsci.4c00140. eCollection 2024 Jun 14.

Backbone

• Vector backbone: pET300NT-DEST
• Backbone manufacturer: Thermofisher
• Backbone size w/o insert (bp): 5693
• Total vector size (bp): 6453
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21 (DE3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pET300-GvpACaM
• Species: H. sapiens (human); H. salinarum
• Insert Size (bp): 760
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: 5′-TAATACGACTCACTATAGGG-3′
• 3′ sequencing primer: 5’-TAGTTATTGCTCAGCGGTGG-3’

Resource Information

• Supplemental Documents:
  • 19ACIHPC_gvpACalmodulinNTHIS.gb
• A portion of this plasmid was derived from a plasmid made by: Thermofisher Geneart Gene Synthesis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene comment: Note that there are some differences between the Addgene verified sequence and the depositor's reference sequence (see supplemental document). These differences do not affect the function of the plasmid.