MFE23-ZZ
(Plasmid #223553)

• Purpose: MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.
• Depositing Lab: Jean Gariepy
• Publication: Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6):1864-1873. doi: 10.1021/acsptsci.4c00140. eCollection 2024 Jun 14.

Backbone

• Vector backbone: pET302NT-his
• Backbone manufacturer: Thermofisher
• Backbone size w/o insert (bp): 5712
• Total vector size (bp): 6953
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21 (DE3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pET302-MFE23-ZZ
• Species: M. musculus (mouse); S. aureus
• Insert Size (bp): 1269
• Promoter: T7
• Tags / Fusion Proteins:
  • HA tag (N terminal on insert)
  • HIS tag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: 5′-TAATACGACTCACTATAGGG-3′
• 3′ sequencing primer: 5’-TAGTTATTGCTCAGCGGTGG-3’

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Thermofisher Geneart Gene Synthesis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene comment: Note that there are some differences between the Addgene verified sequence and the depositor's reference sequence (see supplemental document). These differences do not affect the function of the plasmid.