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pCHMP2A-mut-NS3-Green
(Plasmid #223529)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223529 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA 3.1
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7536
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHMP2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2157
  • Entrez Gene
    CHMP2A (a.k.a. BC-2, BC2, CHMP2, VPS2, VPS2A)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NS3 Hepatitis C protease (C terminal on insert)
    • mNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer pCDNA3_for, CMVfor, pEGFPN1for
  • 3′ sequencing primer pCDNA3_rev, pCR3.1-BGHrev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ThermoFisher gen'art

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCHMP2A-mut-NS3-Green was a gift from Winfried Weissenhorn (Addgene plasmid # 223529 ; http://n2t.net/addgene:223529 ; RRID:Addgene_223529)

  • For your References section:

    An Inducible ESCRT-III Inhibition Tool to Control HIV-1 Budding. Wang H, Gallet B, Moriscot C, Pezet M, Chatellard C, Kleman JP, Gottlinger H, Weissenhorn W, Boscheron C. Viruses. 2023 Nov 22;15(12):2289. doi: 10.3390/v15122289. 10.3390/v15122289 PubMed 38140530
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