pCHMP4B-NS3-Green
(Plasmid
#223528)
-
PurposeMammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 223528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCDNA 3.1
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 7547
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCHMP4B
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2151
-
Entrez GeneCHMP4B (a.k.a. C20orf178, CHMP4A, CTPP3, CTRCT31, SNF7, SNF7-2, Shax1, VPS32B, Vps32-2, dJ553F4.4)
- Promoter CMV
-
Tags
/ Fusion Proteins
- NS3 Hepatitis C protease (C terminal on insert)
- mNeonGreen (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer pCDNA3_for, CMVfor, pEGFPN1for
- 3′ sequencing primer pCDNA3_rev, pCR3.1-BGHrev (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThermoFisher Gen'art
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCHMP4B-NS3-Green was a gift from Winfried Weissenhorn (Addgene plasmid # 223528 ; http://n2t.net/addgene:223528 ; RRID:Addgene_223528) -
For your References section:
An Inducible ESCRT-III Inhibition Tool to Control HIV-1 Budding. Wang H, Gallet B, Moriscot C, Pezet M, Chatellard C, Kleman JP, Gottlinger H, Weissenhorn W, Boscheron C. Viruses. 2023 Nov 22;15(12):2289. doi: 10.3390/v15122289. 10.3390/v15122289 PubMed 38140530