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Addgene

pKanB alpha
(Plasmid #223524)

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Item Catalog # Description Quantity Price (USD)
Plasmid 223524 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pPICZB alpha
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    Insertion of GAP promoter fused to G418 resistance gene into backbone
  • Vector type
    Yeast Expression
  • Promoter AOX1
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKanB alpha was a gift from Geoff Lin-Cereghino (Addgene plasmid # 223524 ; http://n2t.net/addgene:223524 ; RRID:Addgene_223524)

  • For your References section:

    Direct selection of Pichia pastoris expression strains using new G418 resistance vectors. Lin-Cereghino J, Hashimoto MD, Moy A, Castelo J, Orazem CC, Kuo P, Xiong S, Gandhi V, Hatae CT, Chan A, Lin-Cereghino GP. Yeast. 2008 Apr;25(4):293-9. doi: 10.1002/yea.1587. 10.1002/yea.1587 PubMed 18327886
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