pAND-MCS
(Plasmid
#223514)
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Purpose(Empty Backbone) Plasmid backbone with multiple cloning site for two genes in opposing directions (convergently transcribed)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEXT20
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Vector typeBacterial Expression
- Promoter Ptac and Ptet
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
modified from pAND Addgene #49377
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAND-MCS was a gift from Yolanda Schaerli (Addgene plasmid # 223514 ; http://n2t.net/addgene:223514 ; RRID:Addgene_223514) -
For your References section:
A direct experimental test of Ohno’s hypothesis. Ljiljana Mihajlovic, Bharat Ravi Iyengar, Florian Baier, Içvara Barbier, Justyna Iwaszkiewicz, Vincent Zoete, Andreas Wagner, Yolanda Schaerli. eLife13:RP97216 10.7554/eLife.97216.1