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pEVOL_PylRS(AF)-tRNA
(Plasmid #223511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEVOL
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies)
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    2730
  • Mutation
    Y306A; Y384F
  • Entrez Gene
    pylS (a.k.a. MM_RS07465, MM_1445)
  • Promoter araBAD, rrnB, and glnS
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tRNA Pyl
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    72
  • Promoter proK

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVOL_PylRS(AF)-tRNA was a gift from Edward Lemke (Addgene plasmid # 223511 ; http://n2t.net/addgene:223511 ; RRID:Addgene_223511)

  • For your References section:

    Click strategies for single-molecule protein fluorescence. Milles S, Tyagi S, Banterle N, Koehler C, VanDelinder V, Plass T, Neal AP, Lemke EA. J Am Chem Soc. 2012 Mar 21;134(11):5187-95. doi: 10.1021/ja210587q. Epub 2012 Mar 5. 10.1021/ja210587q PubMed 22356317
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