pBAD_FLAG-GFP(Y39TAG)-6His
(Plasmid
#223510)
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PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223510 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP(Y39TAG)
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SpeciesAequorea victoria
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Insert Size (bp)738
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MutationY39TAG
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD_FLAG-GFP(Y39TAG)-6His was a gift from Edward Lemke (Addgene plasmid # 223510 ; http://n2t.net/addgene:223510 ; RRID:Addgene_223510) -
For your References section:
Click strategies for single-molecule protein fluorescence. Milles S, Tyagi S, Banterle N, Koehler C, VanDelinder V, Plass T, Neal AP, Lemke EA. J Am Chem Soc. 2012 Mar 21;134(11):5187-95. doi: 10.1021/ja210587q. Epub 2012 Mar 5. 10.1021/ja210587q PubMed 22356317