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pCMV_NESPylRS(AF)_hU6tRNAPyl
(Plasmid #223508)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223508 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    NES Pyrrolysyl-tRNA Synthetase (AF)
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1392
  • Mutation
    Y306A; Y384F
  • Entrez Gene
    pylS (a.k.a. MM_RS07465, MM_1445)
  • Promoter CVM, SP6

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tRNA Pyl
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    321
  • Promoter U6

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_NESPylRS(AF)_hU6tRNAPyl was a gift from Edward Lemke (Addgene plasmid # 223508 ; http://n2t.net/addgene:223508 ; RRID:Addgene_223508)

  • For your References section:

    Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy. Nikic I, Estrada Girona G, Kang JH, Paci G, Mikhaleva S, Koehler C, Shymanska NV, Ventura Santos C, Spitz D, Lemke EA. Angew Chem Int Ed Engl. 2016 Dec 23;55(52):16172-16176. doi: 10.1002/anie.201608284. Epub 2016 Nov 2. 10.1002/anie.201608284 PubMed 27804198
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