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Addgene

CMV-SNAP-hCB2_IRES_EGFP
(Plasmid #223506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2
  • Backbone manufacturer
    Clontech (TaKaRa)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNAP-tag-CNR2 and EGFP
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    CNR2 (a.k.a. CB-2, CB2, CX5)
  • Tag / Fusion Protein
    • SNAP-tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-SNAP-hCB2_IRES_EGFP was a gift from James Frank (Addgene plasmid # 223506 ; http://n2t.net/addgene:223506 ; RRID:Addgene_223506)

  • For your References section:

    Platform Reagents Enable Synthesis of Ligand-Directed Covalent Probes: Study of Cannabinoid Receptor 2 in Live Cells. Kosar M, Sykes DA, Viray AEG, Vitale RM, Sarott RC, Ganzoni RL, Onion D, Tobias JM, Leippe P, Ullmer C, Zirwes EA, Guba W, Grether U, Frank JA, Veprintsev DB, Carreira EM. J Am Chem Soc. 2023 Jul 19;145(28):15094-15108. doi: 10.1021/jacs.2c13629. Epub 2023 Jul 4. 10.1021/jacs.2c13629 PubMed 37401816
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