CMV-pDisplay-SNAP[C145A]
(Plasmid
#223496)
-
PurposeExpression of dead-mutant SNAP-tags to outer surface in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 223496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDisplay
-
Backbone manufacturerInvitrogen
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namedead-mutant SNAP-tag
-
SpeciesSynthetic
-
MutationSNAP-tag C145A
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CMV-pDisplay-SNAP[C145A] was a gift from James Frank (Addgene plasmid # 223496 ; http://n2t.net/addgene:223496 ; RRID:Addgene_223496) -
For your References section:
Genetically-targeted photorelease of endocannabinoids enables optical control of GPR55 in pancreatic beta-cells. Tobias JM, Rajic G, Viray AEG, Icka-Araki D, Frank JA. Chem Sci. 2021 Sep 15;12(40):13506-13512. doi: 10.1039/d1sc02527a. eCollection 2021 Oct 20. 10.1039/d1sc02527a PubMed 34777770