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Addgene

pET-28b R6CZ24_6H C-terminal
(Plasmid #223435)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223435 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28b

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    R6CZ24 Thiolase
  • Tag / Fusion Protein
    • His6 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28b R6CZ24_6H C-terminal was a gift from Emily Balskus (Addgene plasmid # 223435 ; http://n2t.net/addgene:223435 ; RRID:Addgene_223435)

  • For your References section:

    Gut microbial metabolism of 5-ASA diminishes its clinical efficacy in inflammatory bowel disease. Mehta RS, Mayers JR, Zhang Y, Bhosle A, Glasser NR, Nguyen LH, Ma W, Bae S, Branck T, Song K, Sebastian L, Pacheco JA, Seo HS, Clish C, Dhe-Paganon S, Ananthakrishnan AN, Franzosa EA, Balskus EP, Chan AT, Huttenhower C. Nat Med. 2023 Mar;29(3):700-709. doi: 10.1038/s41591-023-02217-7. Epub 2023 Feb 23. 10.1038/s41591-023-02217-7 PubMed 36823301
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