pET-28b C7H1G6_6H N-terminal
(Plasmid
#223434)
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PurposeC7H1G6 GNAT expression vector with N-terminal His6 tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223434 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28b
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameC7H1G6 GNAT
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-28b C7H1G6_6H N-terminal was a gift from Emily Balskus (Addgene plasmid # 223434 ; http://n2t.net/addgene:223434 ; RRID:Addgene_223434) -
For your References section:
Gut microbial metabolism of 5-ASA diminishes its clinical efficacy in inflammatory bowel disease. Mehta RS, Mayers JR, Zhang Y, Bhosle A, Glasser NR, Nguyen LH, Ma W, Bae S, Branck T, Song K, Sebastian L, Pacheco JA, Seo HS, Clish C, Dhe-Paganon S, Ananthakrishnan AN, Franzosa EA, Balskus EP, Chan AT, Huttenhower C. Nat Med. 2023 Mar;29(3):700-709. doi: 10.1038/s41591-023-02217-7. Epub 2023 Feb 23. 10.1038/s41591-023-02217-7 PubMed 36823301