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Addgene

pYPQAT58
(Plasmid #223430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ-dzCas9-Act3.0
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
  • Tags / Fusion Proteins
    • NLS
    • 3x FLAG

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQAT58 was a gift from Yiping Qi (Addgene plasmid # 223430 ; http://n2t.net/addgene:223430 ; RRID:Addgene_223430)

  • For your References section:

    A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants. Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y. Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081. 10.1093/plcell/koaf081 PubMed 40202185
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