pYPQAT33
(Plasmid
#223405)
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PurposeT-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223405 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYPQ166-ABE8e
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Vector typePlant Expression, CRISPR
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Selectable markerskanamycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2x35s-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
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Tags
/ Fusion Proteins
- NLS
- 3x FLAG
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQAT33 was a gift from Yiping Qi (Addgene plasmid # 223405 ; http://n2t.net/addgene:223405 ; RRID:Addgene_223405) -
For your References section:
A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants. Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y. Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081. 10.1093/plcell/koaf081 PubMed 40202185
