pCI-MOR31-1
(Plasmid
#22337)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22337 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4006
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMOR31-1
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Alt nameMus musculus olfactory receptor 67
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SpeciesM. musculus (mouse)
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Insert Size (bp)981
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GenBank IDAY317748
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Entrez GeneOlfr67 (a.k.a. 3'[b]1, 3'beta1, MOR31-1)
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Tag
/ Fusion Protein
- Rho (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence I sent is a 100% match to AY317748 (a variant of MOR31-1) and is the variant we used in the paper. L178H and Y10D are apparent in other variants.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-MOR31-1 was a gift from Hiroaki Matsunami (Addgene plasmid # 22337 ; http://n2t.net/addgene:22337 ; RRID:Addgene_22337) -
For your References section:
Odor coding by a Mammalian receptor repertoire. Saito H, Chi Q, Zhuang H, Matsunami H, Mainland JD. Sci Signal. 2009 . 2(60):ra9. 10.1126/scisignal.2000016 PubMed 19261596