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Addgene

MT3
(Plasmid #223331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    s1300-3XFLAG
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3HA-EGFP-miniTurboID-OMP25
  • Alt name
    Synj2bp
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1800
  • Entrez Gene
    Synj2bp (a.k.a. Omp25)
  • Promoter CaMV35S
  • Tag / Fusion Protein
    • 3HA-EGFP-miniTurboID (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    paGFP-Omp25 was a gift from David Sabatini (Addgene plasmid #69598)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MT3 was a gift from Xiaohong Zhu (Addgene plasmid # 223331 ; http://n2t.net/addgene:223331 ; RRID:Addgene_223331)

  • For your References section:

    Mapping of cytosol-facing organelle outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells. Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, Song CP, Zhu X. Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan 23. 10.1111/tpj.16641 PubMed 38261530
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