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Addgene

pDestTol2pA2-5.0dand5:EGFP
(Plasmid #223327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2pA2
  • Backbone size w/o insert (bp) 5240
  • Total vector size (bp) 10240
  • Vector type
    zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5.0kb dand5 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5000
  • Entrez Gene
    dand5 (a.k.a. cha)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's QC result found two 1bp idels and a SNP in the dand5 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2pA2-5.0dand5:EGFP was a gift from Hiroyuki Takeda (Addgene plasmid # 223327 ; http://n2t.net/addgene:223327 ; RRID:Addgene_223327)

  • For your References section:

    Reemployment of Kupffer's vesicle cells into axial and paraxial mesoderm via transdifferentiation. Ikeda T, Inamori K, Kawanishi T, Takeda H. Dev Growth Differ. 2022 Apr;64(3):163-177. doi: 10.1111/dgd.12774. Epub 2022 Mar 15. 10.1111/dgd.12774 PubMed 35129208
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