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Addgene

pLenti-CMV-Puro-PNPT1 S484A
(Plasmid #223316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223316 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-CMV-Puro-PNPT1
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Polyribonucleotide Nucleotidyltransferase 1
  • Alt name
    PNPT1
  • Species
    H. sapiens (human)
  • Mutation
    Changed serine 484 to alanine
  • Entrez Gene
    PNPT1 (a.k.a. COXPD13, DFNB70, OLD35, PNPASE, SCA25, old-35)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-Puro-PNPT1 S484A was a gift from Judy Lieberman (Addgene plasmid # 223316 ; http://n2t.net/addgene:223316 ; RRID:Addgene_223316)

  • For your References section:

    Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis. Miao R, Jiang C, Chang WY, Zhang H, An J, Ho F, Chen P, Zhang H, Junqueira C, Amgalan D, Liang FG, Zhang J, Evavold CL, Hafner-Bratkovic I, Zhang Z, Fontana P, Xia S, Waldeck-Weiermair M, Pan Y, Michel T, Bar-Peled L, Wu H, Kagan JC, Kitsis RN, Zhang P, Liu X, Lieberman J. Immunity. 2023 Nov 14;56(11):2523-2541.e8. doi: 10.1016/j.immuni.2023.10.004. Epub 2023 Nov 3. 10.1016/j.immuni.2023.10.004 PubMed 37924812
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