pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE
(Plasmid
#223287)
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PurposeA complete OptoMBP cassette for dissolution of FUS-CHOP condensates.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPBJ
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Vector typeMammalian Expression, Synthetic Biology ; PiggyBAC transposase insertable vector
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Selectable markersiRFP and FusionRed fluorescence
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemyc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.01.16.575860 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE was a gift from Jared Toettcher (Addgene plasmid # 223287 ; http://n2t.net/addgene:223287 ; RRID:Addgene_223287) -
For your References section:
Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag. Brumbaugh-Reed EH, Aoki K, Toettcher JE. bioRxiv 2024.01.16.575860 10.1101/2024.01.16.575860
Map uploaded by the depositor.
