pHR myc-MBP-SspB-iRFP-NLS
(Plasmid
#223286)
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PurposeAn SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
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Selectable markersFusionRed fluorescence
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMyc-MBP-SspB-irFP-NLS
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Insert Size (bp)2547
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.01.16.575860 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR myc-MBP-SspB-iRFP-NLS was a gift from Jared Toettcher (Addgene plasmid # 223286 ; http://n2t.net/addgene:223286 ; RRID:Addgene_223286) -
For your References section:
Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag. Brumbaugh-Reed EH, Aoki K, Toettcher JE. bioRxiv 2024.01.16.575860 10.1101/2024.01.16.575860
Map uploaded by the depositor.
