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pTwist-SARS-CoV-2 Spike-deltaC18 KP.2
(Plasmid #223273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist-CMV-BetaGlobin-WPRE-Neo
  • Backbone manufacturer
    Twist
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Spike KP.2
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Mutation
    See Depositor Comments.
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CAG

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Relevant Mutations: Ins16MPLF, T19I, R21T, deltaL24P25P26,A27S,S50L,deltaH69V70,V127F,G142D,deltaY144,F157S,R158G,deltaN211,L212I,V213G,L216F,H245N,A246D,I332V,G339H,R346T,K356T,S371F,S373P,S375F,T376A,R403K,D405N,R408S,K417N,N440K,V445H,G446S,N450D,L452W,L455S,F456L,N460K,S477NT478K,N481K,V483E,deltaE484,F486P,Q498R,N501Y,Y505H,E554K,A570V,D614G,P621S,H655Y,N679K,P681R,N764K,D796Y,S939F,Q954H,N969K,V1104LP1143L

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist-SARS-CoV-2 Spike-deltaC18 KP.2 was a gift from Alejandro Balazs (Addgene plasmid # 223273 ; http://n2t.net/addgene:223273 ; RRID:Addgene_223273)

  • For your References section:

    Ongoing evolution of SARS-CoV-2 drives escape from mRNA vaccine-induced humoral immunity. Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ, Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran WF, Balazs AB. medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 10.1101/2024.03.05.24303815. 10.1101/2024.03.05.24303815 PubMed 38496628
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