AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP
(Plasmid
#223253)
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PurposeAAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223253 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-hSyn-FLEX (Addgene#50455)
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemacaque OPN4-IRES-EGFP
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GenBank IDOP856641
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Entrez GeneOPN4
- Promoter human Synapsin 1 (hSyn)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byBryan Roth. Original vector: pAAV-hSyn-FLEX (Addgene #50455)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP was a gift from Michael Do (Addgene plasmid # 223253 ; http://n2t.net/addgene:223253 ; RRID:Addgene_223253) -
For your References section:
Encoding of environmental illumination by primate melanopsin neurons. Liu A, Milner ES, Peng YR, Blume HA, Brown MC, Bryman GS, Emanuel AJ, Morquette P, Viet NM, Sanes JR, Gamlin PD, Do MTH. Science. 2023 Jan 27;379(6630):376-381. doi: 10.1126/science.ade2024. Epub 2023 Jan 26. 10.1126/science.ade2024 PubMed 36701440