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Addgene

pEF1a-donor_opt(R2Tg)-GFP(intron)
(Plasmid #223252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    R2Tg donor_opt
  • Species
    Other

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-donor_opt(R2Tg)-GFP(intron) was a gift from Wei Li (Addgene plasmid # 223252 ; http://n2t.net/addgene:223252 ; RRID:Addgene_223252)

  • For your References section:

    All-RNA-mediated targeted gene integration in mammalian cells with rationally engineered R2 retrotransposons. Chen Y, Luo S, Hu Y, Mao B, Wang X, Lu Z, Shan Q, Zhang J, Wang S, Feng G, Wang C, Liang C, Tang N, Niu R, Wang J, Han J, Yang N, Wang H, Zhou Q, Li W. Cell. 2024 Jul 1:S0092-8674(24)00694-9. doi: 10.1016/j.cell.2024.06.020. 10.1016/j.cell.2024.06.020 PubMed 38981481
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