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Addgene

sUPRa
(Plasmid #223242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG-mNeonGreen
  • Backbone manufacturer
    Addgene 99134
  • Backbone size w/o insert (bp) 2896
  • Total vector size (bp) 6151
  • Modifications to backbone
    Removed the CAG promoter and the WPRE.
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    None
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sUPRa
  • Insert Size (bp)
    3255
  • Promoter Custom

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site PmlI (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sUPRa was a gift from Colin Akerman (Addgene plasmid # 223242 ; http://n2t.net/addgene:223242 ; RRID:Addgene_223242)

  • For your References section:

    sUPRa is a dual-color reporter for unbiased quantification of the unfolded protein response with cellular resolution. Chakrabarty A, Newey SE, Promi MM, Agbetiameh BK, Munro D, Brodersen PJN, Gothard G, Mahfooz K, Mengual JP, Vyazovskiy VV, Akerman CJ. Sci Rep. 2024 Jul 1;14(1):14990. doi: 10.1038/s41598-024-65611-2. 10.1038/s41598-024-65611-2 PubMed 38951511
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