pGEX-6p1-GST-Ubiquitin
(Plasmid
#223236)
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PurposeBacterial expression construct for GST-Tagged Ubiquitin production and purification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6p1
- Backbone size w/o insert (bp) 4984
- Total vector size (bp) 5212
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUbiquitin
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SpeciesH. sapiens (human)
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Insert Size (bp)228
- Promoter tac
Cloning Information
- Cloning method Other
- 5′ sequencing primer CCTTTGCAGGGCTGGCAAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6p1-GST-Ubiquitin was a gift from Weixing Zhao (Addgene plasmid # 223236 ; http://n2t.net/addgene:223236 ; RRID:Addgene_223236)