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pAAV-U6-sgRev-erba-hSyn-mCherry-KASH
(Plasmid #223226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-U6-sgRNA-hSyn-mCherry-KASH
  • Backbone size w/o insert (bp) 5270
  • Total vector size (bp) 5293
  • Modifications to backbone
    Destroyed SapI digestion site
  • Vector type
    Mammalian Expression, Mouse Targeting, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nr1d1
  • Alt name
    Rev-erb alpha
  • gRNA/shRNA sequence
    GTTGCGATTGATGCGAACGATGG
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_145434.4
  • Entrez Gene
    Nr1d1 (a.k.a. A530070C09Rik)
  • Promoter U6
  • Tag / Fusion Protein
    • mCherry

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-U6-sgRev-erba-hSyn-mCherry-KASH was a gift from Kyungjin Kim (Addgene plasmid # 223226 ; http://n2t.net/addgene:223226 ; RRID:Addgene_223226)

  • For your References section:

    Role of the circadian nuclear receptor REV-ERBalpha in dorsal raphe serotonin synthesis in mood regulation. Park I, Choi M, Kim J, Jang S, Kim D, Kim J, Choe Y, Geum D, Yu SW, Choi JW, Moon C, Choe HK, Son GH, Kim K. Commun Biol. 2024 Aug 15;7(1):998. doi: 10.1038/s42003-024-06647-y. 10.1038/s42003-024-06647-y PubMed 39147805
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