pHAND1-AM-Tag-PGK-Neo
(Plasmid
#223216)
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PurposeTargeting plasmid for inserting a C-terminal AM-Tag in the human HAND1 gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223216 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2698
- Total vector size (bp) 6286
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Vector typeCre/Lox ; Human Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHAND1
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SpeciesH. sapiens (human)
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Insert Size (bp)3588
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GenBank IDNM_004821.3
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Entrez GeneHAND1 (a.k.a. Hxt, Thing1, bHLHa27, eHand)
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Tag
/ Fusion Protein
- AM-Tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTAAAACGACGGCCAGT
- 3′ sequencing primer CAGGAAACAGCTATGAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAND1-AM-Tag-PGK-Neo was a gift from Matthew Birket (Addgene plasmid # 223216 ; http://n2t.net/addgene:223216 ; RRID:Addgene_223216) -
For your References section:
HAND1 level controls the specification of multipotent cardiac and extraembryonic progenitors from human pluripotent stem cells. Lynch AT, Phillips N, Douglas M, Dorgnach M, Lin IH, Adamson AD, Darieva Z, Whittle J, Hanley NA, Bobola N, Birket MJ. EMBO J. 2025 Mar 31. doi: 10.1038/s44318-025-00409-0. 10.1038/s44318-025-00409-0 PubMed 40164946