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Addgene

pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP
(Plasmid #223214)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223214 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHR
  • Backbone manufacturer
    Ron Vale (Addgene # 60906)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Soluble TNFαR
  • Species
    H. sapiens (human)
  • Entrez Gene
    TNFRSF1A (a.k.a. CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Created with TNFa gblock (WT)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP was a gift from Wendell Lim (Addgene plasmid # 223214 ; http://n2t.net/addgene:223214 ; RRID:Addgene_223214)
  • For your References section:

    Engineering synthetic suppressor T cells that execute locally targeted immunoprotective programs. Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok M, Lim WA. Science. 2024 Dec 6;386(6726):eadl4793. doi: 10.1126/science.adl4793. Epub 2024 Dec 6. 10.1126/science.adl4793 PubMed 39636990