pHR-GAL4-IL10-IRES-mCherry-PGK-BFP
(Plasmid
#223209)
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PurposeLentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Backbone manufacturerRon Vale (Addgene # 60906)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIL10
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SpeciesH. sapiens (human)
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Entrez GeneIL10 (a.k.a. CSIF, GVHDS, IL-10, IL10A, TGIF)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Created with IL10 gblock (WT)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-GAL4-IL10-IRES-mCherry-PGK-BFP was a gift from Wendell Lim (Addgene plasmid # 223209 ; http://n2t.net/addgene:223209 ; RRID:Addgene_223209) -
For your References section:
Engineering synthetic suppressor T cells that execute locally targeted immunoprotective programs. Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok M, Lim WA. Science. 2024 Dec 6;386(6726):eadl4793. doi: 10.1126/science.adl4793. Epub 2024 Dec 6. 10.1126/science.adl4793 PubMed 39636990