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Addgene

pENTR-FLAG-HAND1-T2A-mTagBFP2
(Plasmid #223192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR4-FLAG
  • Backbone manufacturer
    Addgene ID 17423
  • Backbone size w/o insert (bp) 2276
  • Total vector size (bp) 3740
  • Vector type
    Bacterial Expression ; Promoterless entry vector for Gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HAND1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1464
  • GenBank ID
    NM_004821.3
  • Entrez Gene
    HAND1 (a.k.a. Hxt, Thing1, bHLHa27, eHand)
  • Promoter None
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • T2A-mTagBFP2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTACAAACTCTTCCTGTTAGTTAG
  • 3′ sequencing primer ATGGCTCATAACACCCCTTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-FLAG-HAND1-T2A-mTagBFP2 was a gift from Matthew Birket (Addgene plasmid # 223192 ; http://n2t.net/addgene:223192 ; RRID:Addgene_223192)

  • For your References section:

    HAND1 level controls the specification of multipotent cardiac and extraembryonic progenitors from human pluripotent stem cells. Lynch AT, Phillips N, Douglas M, Dorgnach M, Lin IH, Adamson AD, Darieva Z, Whittle J, Hanley NA, Bobola N, Birket MJ. EMBO J. 2025 Mar 31. doi: 10.1038/s44318-025-00409-0. 10.1038/s44318-025-00409-0 PubMed 40164946
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