pCI-OR10J5
(Plasmid
#22318)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4006
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOR10J5
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Alt nameHomo sapiens olfactory receptor, family 10, subfamily J, member 5
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SpeciesH. sapiens (human)
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Insert Size (bp)930
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GenBank IDNM_001004469
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Entrez GeneOR10J5 (a.k.a. MOR23, OLFR16, OR1-28)
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Tag
/ Fusion Protein
- Rho (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-OR10J5 was a gift from Hiroaki Matsunami (Addgene plasmid # 22318 ; http://n2t.net/addgene:22318 ; RRID:Addgene_22318) -
For your References section:
Odor coding by a Mammalian receptor repertoire. Saito H, Chi Q, Zhuang H, Matsunami H, Mainland JD. Sci Signal. 2009 . 2(60):ra9. 10.1126/scisignal.2000016 PubMed 19261596