icLCT
(Plasmid
#223179)
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PurposeNative rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepUC57
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecytosolic intestinal lactase protein fragment
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Alt nameciLCT
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SpeciesR. norvegicus (rat)
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Insert Size (bp)4175
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Entrez GeneLct (a.k.a. Lph)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byOur lab had it synthesized.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Inspired by the paper:
Salehi S, Eckley L, Sawyer GJ, Zhang X, Dong X, Freund JN, Fabre JW. Intestinal lactase as an autologous beta-galactosidase reporter gene for in vivo gene expression studies. Hum Gene Ther. 2009 Jan;20(1):21-30. doi: 10.1089/hum.2008.101. PMID: 20377368.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
icLCT was a gift from Andreas Stuermer (Addgene plasmid # 223179 ; http://n2t.net/addgene:223179 ; RRID:Addgene_223179)