pCambia-dLB-kanR-GFP
(Plasmid #223177)

• Purpose: (Empty Backbone) Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border
• Depositing Lab: Andreas Stuermer
• Publication: Gene Tracking (unpublished)

Backbone

• Vector backbone: pCambia
• Backbone manufacturer: Cambia Foundation
• Backbone size (bp): 8995
• Vector type: Plant Expression ; Ti plasmid for Agrobacterium
• Selectable markers: Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Growth instructions: Can grow in E. coli (high copy) but also in Agrobacterium (low copy), as normal pCambia vectors. Selection is kanamycin-containing LB in E. coli and Agrobacterium
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • pCAMBIA-doubleborder KanR-GFP v2 neat.gb
• A portion of this plasmid was derived from a plasmid made by: Synthetic DNA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Agrobacterium preferentially starts Ti-DNA transfer at the Right Border, and terminates at the Left Border. In a significant number of transfer events, however, the Left Border does not terminate and DNA transfer sequence is continued.

Based upon the paper:
Huang S, Gilbertson LA, Adams TH, Malloy KP, Reisenbigler EK, Birr DH, Snyder MW, Zhang Q, Luethy MH. Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors. Transgenic Res. 2004 Oct;13(5):451-61. doi: 10.1007/s11248-004-1453-3. PMID: 15587269.