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Addgene

pLentiFUCCI(CA)5
(Plasmid #223176)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223176 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLentiRNAGuide_001
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUCCI(CA)5
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiFUCCI(CA)5 was a gift from Neville Sanjana (Addgene plasmid # 223176 ; http://n2t.net/addgene:223176 ; RRID:Addgene_223176)
  • For your References section:

    Transcriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells. Liang WW, Muller S, Hart SK, Wessels HH, Mendez-Mancilla A, Sookdeo A, Choi O, Caragine CM, Corman A, Lu L, Kolumba O, Williams B, Sanjana NE. Cell. 2024 Dec 26;187(26):7637-7654.e29. doi: 10.1016/j.cell.2024.10.021. Epub 2024 Nov 11. 10.1016/j.cell.2024.10.021 PubMed 39532094