pLentiFUCCI(CA)5
(Plasmid
#223176)
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PurposeLentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLentiRNAGuide_001
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFUCCI(CA)5
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLentiFUCCI(CA)5 was a gift from Neville Sanjana (Addgene plasmid # 223176 ; http://n2t.net/addgene:223176 ; RRID:Addgene_223176) -
For your References section:
Transcriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells. Liang WW, Muller S, Hart SK, Wessels HH, Mendez-Mancilla A, Sookdeo A, Choi O, Caragine CM, Corman A, Lu L, Kolumba O, Williams B, Sanjana NE. Cell. 2024 Dec 26;187(26):7637-7654.e29. doi: 10.1016/j.cell.2024.10.021. Epub 2024 Nov 11. 10.1016/j.cell.2024.10.021 PubMed 39532094