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pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
(Plasmid #223173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hfCas13d (N2V8)
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • NLS-HA (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.13.594039 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast was a gift from Neville Sanjana (Addgene plasmid # 223173 ; http://n2t.net/addgene:223173 ; RRID:Addgene_223173)

  • For your References section:

    Low copy CRISPR-Cas13d mitigates collateral RNA cleavage. Hart SK, Wessels HH, Mendez-Mancilla A, Muller S, Drabavicius G, Choi O, Sanjana NE. bioRxiv [Preprint]. 2024 May 14:2024.05.13.594039. doi: 10.1101/2024.05.13.594039. 10.1101/2024.05.13.594039 PubMed 38798586
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