pRS403-PGAL1-strongSC_GFP
(Plasmid #22316)

• Depositing Lab: David Bartel
• Publication: Drinnenberg et al Science. 2009 Sep 10. ():.

Backbone

• Vector backbone: pRS403
• Backbone size w/o insert (bp): 4456
• Vector type: Yeast Expression ; Integrating plasmid
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): SURE
• Growth instructions: Provided in SURE cells (Stratagene)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP-rad9-GFP
• Insert Size (bp): 623

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: CYC1

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GAL1 promoter, GFP silencing construct and CYC1 terminator fragment was cloned out of pYES2.1 and into pRS403.

The insert sequence can be found by clicking on the "View sequence" link. The rad9 intron loop is shown in upper case letters.