pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)
(Plasmid #223152)

• Purpose: Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold
• Depositing Lab: Boris Kantor
• Publication: Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.14.536951. doi: 10.1101/2023.04.14.536951.

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Backbone

• Vector backbone: Unknown
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HP1b
• Alt name: CBX1
• Species: H. sapiens (human)
• Mutation: Encodes aa 79-173
• Entrez Gene: CBX1 (a.k.a. CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, Hp1beta, M31, MOD1, p25beta)
• Promoter: EF1a
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.14.536951 for bioRxiv preprint.