pRS403-PGAL1-weakSC_GFP
(Plasmid #22315)

• Depositing Lab: David Bartel
• Publication: Drinnenberg et al Science. 2009 Sep 10. ():.

Backbone

• Vector backbone: pRS403
• Backbone size w/o insert (bp): 4456
• Vector type: Yeast Expression ; Integrating plasmid
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP-rad9
• Insert Size (bp): 348

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: CYC1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GAL1 promoter, GFP silencing construct and CYC1 terminator fragment was cloned out of pYES2.1 and into pRS403.

Please note that Addgene's sequencing result differs from the full plasmid sequence provided by the depositing laboratory by a single nucleotide mismatch at bp# 2910. This difference is not known to affect plasmid function.