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Addgene

pRS405-PTEF-Dcr1
(Plasmid #22314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS404-PTEF
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Yeast Expression ; Integrating plasmid
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DCR1
  • Species
    Saccharomyces castellii

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DcrI coding sequence was inserted between TEF promoter and CYC1 terminator.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS405-PTEF-Dcr1 was a gift from David Bartel (Addgene plasmid # 22314 ; http://n2t.net/addgene:22314 ; RRID:Addgene_22314)

  • For your References section:

    RNAi in Budding Yeast. Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP. Science. 2009 Sep 10. ():. 10.1126/science.1176945 PubMed 19745116
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