plp
(Plasmid #22310)

• Purpose: (Empty Backbone)
• Depositing Lab: David Bartel
• Publication: Drinnenberg et al Science. 2009 Sep 10. ():.

Backbone

• Vector backbone: pYES2.1 modified
• Backbone size (bp): 5857
• Vector type: Yeast Expression ; Integrating plasmid
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PvuII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GAL1
• 3′ sequencing primer: CYC1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A MCS containing XhoI and EcoRI sites was cloned between the PvuII and XbaI sites of pYES2.1. The empty vector was created by replacing the hairpin of pIp-strongSC_GFP with XhoI and EagI sites

To make this plasmid integrating, the 2micron and f1 origins were replaced (using NheI and SpeI sites with sequence from S. castellii sc633:288301-289016 (amplified from genomic DNA with 5'-AAAAGCTAGCGATCCCTTATCAAATATGGTAC and 5'-AAAAACTAGTGTAGAATCCAGAGAATAGAATC).

Please note that Addgene's sequencing results differ from the full plasmid sequence from the depositing laboratory by a single nucleotide mismatch at bp# 3135 in the vector backbone. This mismatch is not known to affect plasmid function.