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Addgene

pYES2.1-GFP
(Plasmid #22308)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22308 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pYES2.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5857
  • Vector type
    Yeast Expression ; 2micron
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Galactose-inducible expression plasmid.

Note that the map below labels the fluorescent marker as YFP. This designation is a product of the Addgene map algorithm. The depositing lab has confirmed this plasmid as conferring green fluorescence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYES2.1-GFP was a gift from David Bartel (Addgene plasmid # 22308)

  • For your References section:

    RNAi in Budding Yeast. Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP. Science. 2009 Sep 10. ():. 10.1126/science.1176945 PubMed 19745116
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