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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22307 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYES2.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5857
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Vector typeYeast Expression ; 2micron
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDCR1
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SpeciesSaccharomyces castellii
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GAL1
- 3′ sequencing primer CYC1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Galactose-inducible expression plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYES2.1-Dcr1 was a gift from David Bartel (Addgene plasmid # 22307 ; http://n2t.net/addgene:22307 ; RRID:Addgene_22307) -
For your References section:
RNAi in Budding Yeast. Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP. Science. 2009 Sep 10. ():. 10.1126/science.1176945 PubMed 19745116
